ABSTRACT
Objective To investigate the relationship between glutathione S-transferases P1(GSTP1)Ile105Val and glutathione S-transferases M1(GSTM1)single nucleotide polymorphisms(SNP) and the sensitivity to chemotherapy among patients with ad-vanced non-small cell lung cancer(NSCLC) .Methods We used gene sequencing analysis to determine the SNP of GSTP1 Ile105Val and PCR analysis to GSTM1 in DNA from peripheral lymphocytes of NSCLC patients .Totally 89 patients with NSCLC were trea-ted with platinum-based chemotherapy ,and clinical response was evaluated after 2 cycles .The association between GSTP1 Ile105Val and GSTM1 SNP and chemosensitivity were analyzed .Results The overall response rate was 29 .2% .Chemotherapy re-sponse did not show statistically significant differences between the wild genotypes and the variant genotypes for the GSTP1 Ile105Val and GSTM1 gene(P>0 .05) .Conclusion The polymorphisms of GSTP1 Ile105Val and GSTM1 may be not associated with sensitivity to chemotherapy in NSCLC patients .
ABSTRACT
Objective:To investigate the distribution of common pathogens of senile lower respiratory tract infection and drug resistance.to improve clinical therapeutic efficacy.Methods: The susceptibility to antimicrobial agents was tested with diffusion method on mueller-Hinton medium.The double-dish test for ESBLs Confirmed all putative ESBLs-producing strains.Results:A total of 263 strains were obtained from lower respiratory tract in senile patients.108 of 263 strains were Gram-negative bacilli(38.7%).There were 30 strains of Klebsiellae pneumonia,26 strains of Escherichia coli,21 strains of E.cloacae and 17 strains of Acinetobacter;64 strains of gram-positive bacteria(24.3%) and 91 strains of fungi(34.6%).Nineteen strains(33.9%) produced ESBLs in Escherichia coli and Klebsiella pneumoniae.Most gram-negative bacilli kept high susceptibility to the imipenem.Conclusions:Grau-negative bacilli were predominant in aged patients with lower respiratory tract infection.Attention should be paid to multi-drug resistance of pathogenic bacteria and fungus infection.